Expression of Nuclear Factor Kappa P65 ;Ki-67 and Caspase-3 in Chemically -Induced Bladder Carcinogenesis

The increased expression of NF‐κB associated with carcinogenesis resulted in cell proliferation and stop apoptosis,in bladder carcinogenesis induced by dibutylamine / sodium nitrate mediated by e-coli infection. This study was designed to evaluate the potential protective effect of curcum in rats administered nitrosamine precursors; dibutylamine (DBA) and sodium nitrate (NaNO3); and infected with Escherichia coli (E. coli) and also to monitor changes in nuclear factor the Kappa B p65 (NF‐κB p56) pathway and its downstream products, Bcl-2 and interleukin-6 (IL-6), in parallel with nitrosamine precursors, E. Coli and curcum treatment. Rats were divided into three groups (n = 25 each): Group I a normal control group, group II administered DBA/NaNO3 in drinking water and group III was administered DBA/NaNO3 in drinking water, infected with E. coli and receiving standard diet containing 1% curcum powder. and group IV received DBA/NaNO3 +E.coli+ curcum. Dibutylamine was given as 1000 ppm, NaNO3 was given as 2000 ppm in drinking water, infected by 0.1 ml saline containing suspension of E. coli in the bladder (approximately 2 X 106 organisms) and received standard diet containing 1% curcum powder mixed in the diet. Histopathological examination and immunohistochemical studies reflected that the curcum treated group featured a lower incidence of urinary bladder lesions, and lower levels of NF‐κB, Bcl‐2 and IL‐6 and Ki67 protein than the group receiving nitrosamine precursor and infected with E. coli. These findings suggested that curcum may have a protective role during the process of bladder carcinogenesis by inhibiting the NF‐κB pathway and its downstream products. keywords: Bladder carcinogenesis; Curcum; E.coli; DBA; NF‐κBp65, Ki67; Immunohistochemistry; Caspase-3.